A Dual-Carbon-and-Nitrogen Stable Isotope Ratio Model Is Not Superior to a Single-Carbon Stable Isotope Ratio Model for Predicting Added Sugar Intake in Southwest Virginian Adults

卷:145

Hedrick, Valisa E. ; Zoellner, Jamie M. ; Jahren, A. Hope ; Woodford, Natalie A. ; Bostic, Joshua N. ; Davy, Brenda M.

关键词: added sugars;    biomarker validation;    dietary assessment;    obesity;    sugar-sweetened beverages;   

DOI  :  10.3945/jn.115.211011

学科分类：食品科学和技术

【 摘 要 】

Background: An objective measure of added sugar (AS) and sugar-sweetened beverage (SSB) intake is needed. The delta C-13 value of finger-stick blood is a novel validated biomarker of AS/SSB intake; however, nonsweetener corn products and animal protein also carry a delta C-13 value similar to AS sources, which may affect blood delta C-13 values. The delta N-15 value of blood has been proposed as a "correction factor" for animal protein intake. Objectives: The objectives were to 1) identify foods associated with delta C-13 and delta N-15 blood values, 2) determine the contribution of nonsweetener corn to the diet relative to AS intake, and 31 determine if the dual-isotope model (delta C-13 and delta N-15) is a better predictor of AS/SSB intake than delta C-13 alone. Methods: A cross-sectional sample of southwest Virginian adults In = 257; aged 42 +/- 15 y; 74% overweight/obese) underwent dietary intake assessments and provided finger-stick blood samples, which were analyzed for delta C-13 and delta N-15 values by using natural abundance stable isotope mass spectrometry. Statistical analyses included ANOVAs, paired-samples t tests, and multiple linear regressions. Results: The mean +/- SD daily AS intake was 88 +/- 59 g and nonsweetener corn intake was 13 +/- 13 g. The mean delta C-13 value was -19.1 +/- 0.9 parts per thousand, which was significantly correlated with AS and SSB intakes (r = 0.32 and 0.39, respectively; P <= 0.01). The delta C-13 value and nonsweetener corn intake and the delta N-15 value and animal protein intake were not correlated. AS intake was significantly greater than nonsweetener corn intake (mean difference = 76.2 +/- 57.2 g; P <= 0.001). The delta C-13 value was predictive of AS/SSB intake (beta range: 0.28-0.35; P <= 0.01); however, delta N-15 was not predictive and minimal increases in R-2 values were observed when the delta N-15 value was added to the model. Conclusions: The data do not provide evidence that the dual-isotope method is superior for predicting AS/SSB intakes within a southwest Virginian population. Our results support the potential of the delta C-13 value of finger-stick blood to serve as an objective measure of AS/SSB intake. This trial was registered at clinicaltrials.gov as NCT02193009.