Identification and simultaneous quantification of five alkaloids in Piper longum L. by HPLC-ESI-MSn and UFLC-ESI-MS/MS and their application to Piper nigrum L.

卷:177

Liu, Hao-Long ; Luo, Rong ; Chen, Xiao-Qing ; Ba, Yin-Ying ; Zheng, Li ; Guo, Wei-Wei ; Wu, Xia

关键词: Piper longum L.;    P. nigrum L.;    Alkaloids;    Quantification;    HPLC-ESI-MSn;    UFLC-ESI-MS/MS;   

DOI  :  10.1016/j.foodchem.2015.01.033

学科分类：食品科学和技术

【 摘 要 】

A simple, effective and suitable UFLC-ESI-MS/MS method was firstly developed to simultaneously determine five characteristic constituents (piperine, piperlonguminine, Delta alpha,beta-dihydropiperlonguminine, pellitorine and piperanine) of Piper longum L. The total alkaloids of P. longum L. was prepared. The alkaloid contents of Piper nigrum L. and P. longum L were compared. The analysis was carried out in multiple reaction monitoring scan mode. The method showed a good specificity, linearity (R-2 > 0.995), stability (RSD < 2.53%), repeatability (RSD < 2.58%), and recovery (90.0-103.5%). The limits of detection and limits of quantification of five alkaloids were in the range of 0.02-0.03 and 0.05-0.10 ng/mL, respectively. The intra- and inter-day precision was less than 9.30% and 9.55%, respectively. The validation results confirmed that the method could simultaneously determine the target alkaloids in the sample. Furthermore, the identities of the alkaloids were verified by HPLC-ESI-MS/MS. Compared with P. nigrum, P. longum had lower piperine content but was enriched in the other four alkaloids. (C) 2015 Elsevier Ltd. All rights reserved.