Neuroprotective effect of oral choline administration after global brain ischemia in rats

卷:18

Borges, Andrea Aurelio ; El-Batah, Philipe Nicolas ; Yamashita, Lilia Fumie ; Santana, Aline dos Santos ; Lopes, Antonio Carlos ; Freymuller-Haapalainen, Edna ; Coimbra, Cicero Galli ; Sinigaglia-Coimbra, Rita

关键词: Choline;    Toxicity;    Global brain ischemia;    Lipid peroxidation;    Membrane;    Neurodegeneration;    Neuroprotection;    Rat;   

DOI  :  10.1179/1476830514Y.0000000125

学科分类：食品科学和技术

【 摘 要 】

Choline - now recognized as an essential nutrient - is the most common polar group found in the outer leaflet of the plasma membrane bilayer. Brain ischemia-reperfusion causes lipid peroxidation triggering multiple cell death pathways involving necrosis and apoptosis. Membrane breakdown is, therefore, a major pathophysiologic event in brain ischemia. The ability to achieve membrane repair is a critical step for survival of ischemic neurons following reperfusion injury. The availability of choline is a rate-limiting factor in phospholipid synthesis and, therefore, may be important for timely membrane repair and cell survival. This work aimed at verifying the effects of 7-day oral administration with different doses of choline on survival of CA1 hippocampal neurons following transient global forebrain ischemia in rats. The administration of 400 mg/kg/day divided into two daily doses for 7 consecutive days significantly improved CA1 pyramidal cell survival, indicating that the local availability of this essential nutrient may limit postischemic neuronal survival.