Simultaneous determination of 38 veterinary antibiotic residues in raw milk by UPLC-MS/MS

卷:181

Han, R. W. ; Zheng, N. ; Yu, Z. N. ; Wang, J. ; Xu, X. M. ; Qu, X. Y. ; Li, S. L. ; Zhang, Y. D. ; Wang, J. Q.

关键词: Veterinary antibiotics;    Multiclass analysis;    Raw milk;    UPLC MS/MS;   

DOI  :  10.1016/j.foodchem.2015.02.041

学科分类：食品科学和技术

【 摘 要 】

A selective and rapid method has been developed to determine, simultaneously, 38 veterinary antibiotic residues in raw milk by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). One milliliter of raw milk was diluted with 0.5 mL water and 3 mL acetonitrile, then purified using an Oasis HLB cartridge. The eluates were evaporated by nitrogen drying and then reconstituted to 4 mL with water/acetonitrile (8:1) before being injected into the UPLC-MS/MS system. The results indicated recoveries of 68-118% for 14 beta-lactams, 79-118% for eight quinolones, 71-106% for eight sulfonamides, 76-116% for four tetracyclines, 78-106% for three macrolides, and 88-103% for one lincosamides, with coefficients of variation less than 15% for intraday and interday precisions. The limit of quantification for all antibiotics was 0.03-10 mu g kg(-1). This methodology was then applied to field-collected real raw milk samples and trace levels of four antibiotics were detected. (C) 2015 Elsevier Ltd. All rights reserved.