卷:182 | |
Ethylene-dependent regulation of an alpha-L-arabinofuranosidase is associated to firmness loss in 'Gala' apples under long term cold storage | |
Storch, Tatiane Timm ; Finatto, Taciane ; Pegoraro, Camila ; Dal Cero, Joceani ; Laurens, Francois ; Rombaldi, Cesar Valmor ; Quecini, Vera ; Girardi, Cesar Luis | |
关键词: 1-Methylcyclopropene; alpha-L-Arabinofuranosidase; Cell wall; Gene expression; Mains x domestica; Softening; | |
DOI : 10.1016/j.foodchem.2015.02.123 | |
学科分类:食品科学和技术 | |
【 摘 要 】
Fruit texture changes impair the quality of apples submitted to long term storage, especially under cold. The changes are due to cell wall modifications during ripening and senescence and are associated to ethylene. We have investigated the activity of alpha-L-arabinofuranosidase, a glycosyl hydrolase acting on the side chains of pectin in the cell wall and middle lamella. The transcription of arabinofuranosidase coding sequences 1 and 3 was investigated in plant organs and in response to ethylene, employing hormone application and 1-methylcyclopropene. The transcription of arabinofuranosidase genes is not restricted to fruits, although upregulated by ripening and ethylene. Transcripts of the genes were detected under cold storage up to 180 days. Similarly, arabinofuranosidase activity increased with rising levels of ethylene and under cold storage. Levels of ARABINOPURANOSIDASE3 transcripts were higher than those of ARABINOFURANOSIDASE1 suggesting that the first is an important contributor to enzyme activity and texture changes during cold storage. (C) 2015 Elsevier Ltd. All rights reserved.
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