Role of adenosine 5 '-monophosphate-activated protein kinase in alpha-linolenic acid-induced intestinal lipid metabolism

卷:114

Zhou, Xihong ; Chen, Jingqing ; Wu, Weiche ; Wang, Xinxia ; Wang, Yizhen

关键词: alpha-Linolenic acid;    AMP-activated protein kinase;    Lipid metabolism;    Intestine;   

DOI  :  10.1017/S0007114515002391

学科分类：食品科学和技术

【 摘 要 】

n-3 Long-chain PUFA up-regulate intestinal lipid metabolism. However, whether these metabolic effects of PUFA on intestine are mediated by AMP-activated protein kinase (AMPK) remains to be elucidated. To determine the effects of -linolenic acid (ALA) on intestinal fatty acid (FA) metabolism and whether these effects were affected by AMPK deletion, mice deficient in the catalytic subunit of AMPK1 or AMPK2 and wild-type (WT) mice were fed either a high-fat diet (HF) or HF supplemented with ALA (HF-A). The results showed that ALA supplementation decreased serum TAG content in WT mice. ALA also increased mRNA expression of genes (carnitine palmitoyltransferase 1a, acyl-CoA oxidase 1, medium-chain acyl-CoA dehydrogenase, cytochrome P450 4A10 and pyruvate dehydrogenase kinase isoenzyme 4a) involved in intestinal lipid oxidation and mRNA expression of TAG synthesis-related genes (monoacylglycerol O-acyltransferase 2, diacylglycerol O-acyltransferases 1 and 2) in WT mice. Consistent with these, expression levels of phosphorylated AMPK1 and AMPK2 were also increased in WT mice after ALA addition. However, in the absence of either AMPK1 or AMPK2, ALA supplementation failed to increase intestinal lipid oxidation. In addition, no significant effects of either diet (HF and HF-A) or genotype (WT, AMPK1(-/-) and AMPK2(-/-)) on FA uptake in the intestine and faecal TAG output were observed. Our results suggest that AMPK is indispensable for the effects of ALA on intestinal lipid oxidation.