Analysis of bioactivities and chemical composition of Ziziphus joazeiro Mart. using HPLC-DAD

卷:186

Brito, Sharlene M. O. ; Coutinho, Henrique D. M. ; Talvani, Andre ; Coronel, Cathia ; Barbosa, Andreza G. R. ; Vega, Celeste ; Figueredo, Fernando G. ; Tintino, Saulo R. ; Lima, Luciene F. ; Boligon, Aline A. ; Athayde, Margareth L. ; Menezes, Irwin R. A.

关键词: Ziziphus joazeiro;    Chemical profile;    Antibiotic activity;    Anti-Trypanosoma cruzi activity and anti-Leishmania activity;    Antioxidant activity;   

DOI  :  10.1016/j.foodchem.2014.10.031

学科分类：食品科学和技术

【 摘 要 】

The aim of this study was to evaluate the chemical profile and antioxidant, antimicrobial and antiparasitic activities of the hydroalcoholic extract of the leaves of Ziziphus joazeiro Mart. (HELZJ). The antioxidant DPPH and FRAP assays and chemical profile were determined by colorimetric methods and HPLC/DAD. The antiparasitic, antibiotic and antibiotic-modifying activity were evaluated by microdilution assays. The HPLC-DAD assay showed the presence of mostly tannins and flavonoids, such as caffeic acid and quercetin. The levels of polyphenols and flavonoids were 183.136 mg/g extract and 7.37 mg/g extract, respectively. DPPH and FRAP showed low antioxidant activity for the extract. The antibacterial and antifungal activities were not of clinical relevance, showing MIC > 1024 mu g/mL. However, synergism was observed between HELZJ and the antibiotics amikacin and gentamicin, which resulted in decreased bacterial drug resistance. EHFZJ showed low toxicity in fibroblasts in vitro, while antiparasitic results. against Trypnosoma cruzi, Leishmania braziliensis and Leishmania infantum were not clinically relevant. Thus, our results indicate that Z. joazeiro Mart. (HELZI) could be a source of plant-derived natural products that could lead to the development of promising new antibiotic compounds for infectious diseases. (C) 2014 Elsevier Ltd. All rights reserved.