Red palm oil-supplemented and biofortified cassava gari increase the carotenoid and retinyl palmitate concentrations of triacylglycerol-rich plasma in women

卷:35

Zhu, Chenghao ; Cai, Yimeng ; Gertz, Erik R. ; La Frano, Michael R. ; Burnett, Dustin J. ; Burri, Betty J.

关键词: Biofortified cassava;    Red palm oil;    Vitamin A;    beta-Carotene;    Human;   

DOI  :  10.1016/j.nutres.2015.08.003

学科分类：食品科学和技术

【 摘 要 】

Boiled biofortified cassava containing beta-carotene can increase retinyl palmitate in triacylglycerol-rich plasma. Thus, it might alleviate vitamin A deficiency. Cassava requires extensive preparation to decrease its level of cyanogenic glucosides, which can be fatal. Garification is a popular method of preparing cassava that removes cyanogen glucosides. Our objective was to compare the effectiveness of biofortified gari to gari prepared with red palm oil. The study was a randomized crossover trial in 8 American women. Three gari preparations separated by 2-week washout periods were consumed. Treatments (containing 200-225.9 g gari) were as follows: biofortified gari (containing 1 mg beta-carotene), red palm oil fortified gari (1 mg beta-carotene), and unfortified gari with a 0.3-mg retinyl palmitate reference dose. Blood was collected 6 times from -0.5 to 9.5 hours after ingestion. Triacylglycerol-rich plasma was separated by ultracentrifugation and analyzed by high-performance liquid chromatography (HPLC) with diode array detection. Area under the curve for beta-carotene, alpha-carotene, and retinyl palmitate increased after the fortified meals were fed (P < .05), although the retinyl palmitate increase induced by the red palm oil treatment was greater than that induced by the biofortified treatment (P < .05). Vitamin A conversion was 2.4 +/- 0.3 and 4.2 +/- 1.5 mu g pro-vitamin A carotenoid/1 mu g retinol (means +/- SEM) for red palm oil and biofortified gari, respectively. These results show that both treatments increased beta-carotene, alpha-carotene, and retinyl palmitate in triacylglycerol-rich plasma concentrations in healthy well-nourished adult women, supporting our hypothesis that both interventions could support efforts to alleviate vitamin A deficiency. Published by Elsevier Inc.