Fast determination of biogenic amines in beverages by a core-shell particle column

卷:187

Preti, Raffaella ; Antonelli, Marta Letizia ; Bernacchia, Roberta ; Vinci, Giuliana

关键词: Core-shell particle column;    Biogenic amines;    Dansyl chloride;    Wine;    Fruit nectar;    HPLC;   

DOI  :  10.1016/j.foodchem.2015.04.075

学科分类：食品科学和技术

【 摘 要 】

A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core-shell particle column (100 mm x 4.6 mm, 2.6 mu m particle size) has been employed and the biogenic amines were identified and quantified in a total run time of 13 min with ultraviolet (UV) or fluorescence detection (FLD). Chromatographic conditions such as column temperature (kept at 50 degrees C), gradient elution and flow rate have been optimized and the method has been tested on red wine and fruit nectar. The proposed method is enhanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method as to be comparable to UHPLC methods. Green and cost-effective, this method can be used as a quality-control tool for routine quantitative analysis of biogenic amines in beverages for the average laboratory. (C) 2015 Elsevier Ltd. All rights reserved.