Dual-labeled time-resolved fluoroimmunoassay for simultaneous detection of clothianidin and diniconazole in agricultural samples

卷:192

Sheng, Enze ; Shi, Haiyan ; Zhou, Liangliang ; Hua, Xiude ; Feng, Lu ; Yu, Tong ; Wang, Minghua

Nanjing Agr Univ

关键词: Clothianidin;    Diniconazole;    Europium;    Samarium;    Antibody;    Time-resolved fluoroimmunoassay;   

DOI  :  10.1016/j.foodchem.2015.07.023

学科分类：食品科学和技术

【 摘 要 】

Europium (Eu3+) and samarium (Sm3+) were used as fluorescent labels to develop a highly sensitive dual-labeled time-resolved fluoroimmunoassay (TRFIA) for detect clothianidin and diniconazole in food samples. Under the optimized assay conditions, 50% inhibition concentration (IC50) and the limit of detection (LOD, IC10) of clothianidin were 5.08 and 0.021 mu g/L, and 13.14 and 0.029 mu g/L for diniconazole. The cross-reactivities (CRs) were negligible except dinotefuran (9.4%) and uniconazole (4.28%). The recoveries of clothianidin and diniconazole ranged from 79.3% to 108.7% in food samples. The results of TRFIA for the authentic samples were validated by gas chromatography (GC) analyses, and a satisfactory correlations were obtained. These results indicated that the method was an alternative tool for simultaneous detection of clothianidin and diniconazole in food samples. (C) 2015 Elsevier Ltd. All rights reserved.