An integrated approach with experimental and computational tools outlining the cooperative binding between 2-phenylchromone and human serum albumin

卷:196

Caruso, Icaro Putinhon ; Barbosa Filho, Jose Maria ; de Araujo, Alexandre Suman ; de Souza, Fatima Pereira ; Fossey, Marcelo Andres ; Cornelio, Marinonio Lopes

UNESP

关键词: 2-Phenylchromone;    Human serum albumin;    Fluorescence spectroscopy;    Binding density function;    Cooperative binding;    Computational methods;   

DOI  :  10.1016/j.foodchem.2015.10.027

学科分类：食品科学和技术

【 摘 要 】

2-Phenylchromone (2PHE) is a flavone, found in cereals and herbs, indispensable in the human diet. Its chemical structure is the basis of all flavonoids present in black and green tea, soybean, red fruits and so on. Although offering such nutritional value, it still requires a molecular approach to understand its interactions with a specific target. The combination of experimental and computational techniques makes it possible to describe the interaction between 2PHE and human serum albumin (HSA). Fluorescence spectroscopy results show that the quenching mechanism is static, and thermodynamic analysis points to an entropically driven complex. The binding density function method provides information about a positive cooperative interaction, while drug displacement experiments indicate Sites 1 and 2 of HSA as the most probable binding sites. From the molecular dynamic study, it appears that the molecular docking is in agreement with experimental data and thus more realistic. (C) 2015 Elsevier Ltd. All rights reserved.