Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a beta-galactosidase

卷:196

Chen, Jun-ming ; Ding, Li ; Sui, Xiao-chen ; Xia, Yong-mei ; Wan, Hui-da ; Lu, Tong

Jiangnan Univ

关键词: Steviolbioside;    Stevioside;    Hydrolysis;    beta-Galactosidase;    Reaction coupling separation;    Anti-cancer;   

DOI  :  10.1016/j.foodchem.2015.09.035

学科分类：食品科学和技术

【 摘 要 】

A beta-galactosidase from Kluyveromyces lactis was found to specifically catalyze hydrolysis of the glycosyl ester linkage of stevioside to yield steviolbioside, a rare sweetener that also exists in Stevia rebaudiana leaves. In a packed bed reactor, a reaction coupling separation was realized and a production yield of steviolbioside reached 90% in 6 h. The hydrolysis product steviolbioside presented higher cytoxicity on human normal cells (hepatocytes cell L02 and intestinal epithelial cell T84) than stevioside did. Comparing to the typical chemotherapy agent, 5-fluorouracil (5-FU), steviolbioside presents much lower cytotoxicity on all assayed human normal cells; it presented notable inhibition on human hepatacarcinoma cell Hep3B, human breast cancer cell MDA-MB-231 and human pancreatic cancer cell BxPC-3. The remarkable inhibition on MDA-MB-231 cells makes steviolbioside a potential remedy for human breast cancer, when steviolbioside is served as a natural sweetener. (C) 2015 Elsevier Ltd. All rights reserved.