Green tea polyphenol extract in vivo attenuates inflammatory features of neutrophils from obese rats

卷:55

Albuquerque, K. F. F. S. ; Marinovic, M. P. ; Morandi, A. C. ; Bolin, A. P. ; Otton, R.

Univ Sao Paulo

关键词: Obesity;    Immune system;    Polyphenols;    Reactive oxygen species;    Flavonoids;    Catechins;   

DOI  :  10.1007/s00394-015-0940-z

学科分类：食品科学和技术

【 摘 要 】

Our study aimed to evaluate whether obesity induced by cafeteria diet changes the neutrophil effector/inflammatory function and whether treatment with green tea extract (GT) can improve neutrophil function. Male Wistar rats were treated with GT by gavage (12 weeks/5 days/week; 500 mg/kg of body weight), and obesity was induced by cafeteria diet (8 weeks). Neutrophils were obtained from the peritoneal cavity (injection of oyster glycogen). The following analyses were performed: phagocytic capacity, chemotaxis, myeloperoxidase activity (MPO), hypochlorous acid (HOCl), superoxide anion (O (2) (center dot-) ), hydrogen peroxide (H2O2), IL-1 beta, IL-6 and TNF alpha, mRNA levels of inflammatory genes, calcium mobilisation, activities of antioxidant enzymes, hexokinase and G6PDH. Neutrophils from obese rats showed a significant decrease in migration capacity, H2O2 and HOCl production, MPO activity and O (2) (center dot-) production. Phagocytosis and CD11b mRNA levels were increased, while inflammatory cytokines release remained unmodified. mRNA levels of TLR4 and I kappa K were enhanced. Treatment of obese rats with GT increased neutrophil migration, MPO activity, H2O2, HOCl and O (2) (center dot-) production, whereas TNF-alpha and IL-6 were decreased (versus obese). Similar reductions in TLR4, I kappa K and CD11b mRNA were observed. Catalase and hexokinase were increased by obesity, while SOD and G6PDH were decreased. Treatment with GT reduced catalase and increased the GSH/GSSG ratio. In response to a cafeteria diet, we found a decreased chemotaxis, H2O2 release, MPO activity and HOCl production. We also showed a significant immunomodulatory effect of GT on the obese condition recovering some of these factors such H2O2 and HOCl production, also reducing the levels of inflammatory cytokines.