Optimization of ultrasound-assisted extraction to obtain mycosterols from Agaricus bisporus L. by response surface methodology and comparison with conventional Soxhlet extraction

卷:197

Heleno, Sandrina A. ; Diz, Patricia ; Prieto, M. A. ; Barros, Lillian ; Rodrigues, Alirio ; Barreiro, Maria Filomena ; Ferreira, Isabel C. F. R.

Polytech Inst Braganca

关键词: Agaricus bisporus L.;    Ergosterol;    Soxhlet extraction;    Ultrasound-assisted extraction;    Response surface methodology;    Saponification;   

DOI  :  10.1016/j.foodchem.2015.11.108

学科分类：食品科学和技术

【 摘 要 】

Ergosterol, a molecule with high commercial value, is the most abundant mycosterol in Agaricus bisporus L. To replace common conventional extraction techniques (e.g. Soxhlet), the present study reports the optimal ultrasound-assisted extraction conditions for ergosterol. After preliminary tests, the results showed that solvents, time and ultrasound power altered the extraction efficiency. Using response surface methodology, models were developed to investigate the favourable experimental conditions that maximize the extraction efficiency. All statistical criteria demonstrated the validity of the proposed models. Overall, ultrasound-assisted extraction with ethanol at 375 W during 15 min proved to be as efficient as the Soxhlet extraction, yielding 671.5 +/- 0.5 mg ergosterol/100 g dw. However, with n-hexane extracts with higher purity (mg ergosterol/g extract) were obtained. Finally, it was proposed for the removal of the saponification step, which simplifies the extraction process and makes it more feasible for its industrial transference. (C) 2015 Elsevier Ltd. All rights reserved.