Polydiacetylene/triblock copolymer nanoblend applied as a sensor for micellar casein: A thermodynamic approach

卷:197

de Souza, Luana Cypriano ; Rezende, Jaqueline de Paula ; dos Santos Pires, Ana Clarissa ; Mendes da Silva, Luis Henrique ; Hespanhol da Silva, Maria do Carmo ; Castellon Castrillon, Elkin Dario ; de Andrade, Nelio Jose

Univ Fed Vicosa

关键词: Calcium phosphate;    Pentacosadiynoic acid;    L64;    F68;    Interaction enthalpy;    Zeta potential;   

DOI  :  10.1016/j.foodchem.2015.11.071

学科分类：食品科学和技术

【 摘 要 】

Polydiacetylene (PDA) and triblock copolymer nanoblends were synthesized to detect micellar casein (MC), the main milk protein and an indicator of milk quality. UV-Vis spectrum showed that MC induced blue-to-red transition in nanoblends. When nanoblends and MC were separated by dialysis membrane colorimetric response (CR) was similar, whereas a remarkable CR reduction was noticed after addition of dialyzed-MC, suggesting that small molecules present in MC (salts) caused PDA color change. Interaction enthalpy variation between nanoblends and MC showed an abrupt increase that coincided with MC concentration when colorimetric transition occurred. Copolymer hydrophobic/hydrophilic balance and presence of other molecules in the system affected nanoblends CR. MC salts were found to interact with nanoblends leading to color changes. MC concentration, MC salt release, copolymer hydrophobic/hydrophilic balance, and presence of other molecules in the system affected responses of the sensors. These results contribute to future applications of PDA/copolymer nanosensors to dairy models. (C) 2015 Elsevier Ltd. All rights reserved.