Development and validation of an indirect competitive enzyme-linked immunosorbent assay for monitoring organoarsenic compounds in edible chicken and pork and feed

卷:197

Peng, Dapeng ; Feng, Liang ; Pan, Yuanhu ; Wang, Yulian ; Chen, Dongmei ; Wang, Juan ; Yuan, Zonghui

Huazhong Agr Univ

关键词: Organoarsenic compounds;    Monoclonal antibody;    Indirect competitive enzyme linked;    immunosorbent assay;    Edible chicken and pork;    Feed;   

DOI  :  10.1016/j.foodchem.2015.11.055

学科分类：食品科学和技术

【 摘 要 】

For the first time in this study, we used molecular modelling to design a suitable hapten (arsanilic acid, ASA) and produced a broad-specificity monoclonal antibody (mAb). This mAb exhibited the IC50 for ASA was 913.7 mu g L (1) and showed the cross-reactivity to ASA (100%), carbarsone (849.2%), and nitarsone (1159.5%), respectively. Based on this mAb, an optimised indirect competitive enzyme linked immunosorbent assay (ic-ELISA) protocol was developed to monitor organoarsenic compounds (OAs) in edible chicken and pork and feed, which the detection limit for OAs in a muscle matrix ranged from 74.2 mu g kg (1) to 143.3 mu g kg (1) and in a feed matrix ranged from 7.4 mg kg (1) to 11.8 mg kg (1), the recoveries were 61.3-109.6% with a coefficient of variation of less than 10.8%. These data demonstrated that the developed ic-ELISA is a reliable and useful tool for screening OAs in edible chicken and pork and feed. (C) 2015 Elsevier Ltd. All rights reserved.