A simple and selective method for determination of phthalate biomarkers in vegetable samples by high pressure liquid chromatography-electrospray ionization-tandem mass spectrometry

卷:200

Zhou, Xi ; Cui, Kunyan ; Zeng, Feng ; Li, Shoucong ; Zeng, Zunxiang

Sun Yat Sen Univ

关键词: Phthalate monoester metabolites;    Ultrasonic extraction;    Liquid-liquid extraction;    Solid-phase extraction;    Vegetable;    High pressure liquid chromatographyelectrospray ionisation-tandem mass spectrometry;   

DOI  :  10.1016/j.foodchem.2016.01.008

学科分类：食品科学和技术

【 摘 要 】

In the present study, solid-phase extraction cartridges including silica reversed-phase Isolute C-18, polymeric reversed-phase Oasis HLB and mixed-mode anion-exchange Oasis MAX, and liquid-liquid extractions with ethyl acetate, n-hexane, dichloromethane and its mixtures were compared for clean-up of phthalate monoesters from vegetable samples. Best recoveries and minimised matrix effects were achieved using ethyl acetate/n-hexane liquid-liquid extraction for these target compounds. A simple and selective method, based on sample preparation by ultrasonic extraction and liquid-liquid extraction clean-up, for the determination of phthalate monoesters in vegetable samples by liquid chromatography/electrospray ionisation-tandem mass spectrometry was developed. The method detection limits for phthalate monoesters ranged from 0.013 to 0.120 ng g (1). Good linearity (r(2) > 0.991) between MQLs and 1000 x MQLs was achieved. The intra-and inter-day relative standard deviation values were less than 11.8%. The method was successfully used to determine phthalate monoester metabolites in the vegetable samples. (C) 2016 Elsevier Ltd. All rights reserved.