A High-Saturated-Fat, High-Sucrose Diet Aggravates Bone Loss in ovariectomized Female Rats

卷:146

Dong, Xiao-Li ; Li, Chun-Mei ; Cao, Si-Si ; Zhou, Li-Ping ; Wong, Man-Sau

Shenzhen Key Lab Food Biol Safety Control

关键词: high-saturated-fat diet;    high-sucrose diet;    estrogen deficiency;    bone loss;    oxidative stress;    Pparg;   

DOI  :  10.3945/jn.115.225474

学科分类：食品科学和技术

【 摘 要 】

Background: Estrogen deficiency in women and high-saturated fat, high-sucrose (HFS) diets have both been recognized as risk factors for metabolic syndrome. Studies on the combined actions of these 2 detrimental factors on the bone in females are limited. Objective: We sought to determine the interactive actions of estrogen deficiency and an HFS diet on bone properties and to investigate the underlying mechanisms. Methods: Six-month-old Sprague Dawley sham or ovariectomized (OVX) rats were pair fed the same amount of either a low-saturated-fat, low-sucrose (LFS) diet (13% fat calories; 15% sucrose calories) or an HFS diet (42% fat calories; 30% sucrose calories) for 12 wk. Blood, liver, and bone were collected for correspondent parameters measurement. Results: Ovariectomy decreased bone mineral density in the tibia head (TH) by 62% and the femoral end (FE) by 49% (P < 0.0001). The HFS diet aggravated bone loss in OVX rats by an additional 41% in the TH and 37% in the FE (P < 0.05). Bone loss in the HFS-OVX rats was accompanied by increased urinary deoxypyridinoline concentrations by 28% (P < 0.05). The HFS diet induced cathepsin K by 145% but reduced osteoprotegerin mRNA expression at the FE of the HFS-sham rats by 71 % (P < 0.05). Ovariectomy significantly increased peroxisome proliferator-activated receptor gamma mRNA expression by 136% and 170% at the FE of the LFS- and HFS-OVX rats, respectively (P < 0.05). The HFS diet aggravated ovariectomy-induced lipid deposition and oxidative stress (OS) in rat livers (P < 0.05). Trabecular bone mineral density at the FE was negatively correlated with rat liver malondialdehyde concentrations (R-2 = 0.39; P < 0.01). Conclusions: The detrimental actions of the HES diet and ovariectomy on bone properties in rats occurred mainly in cancellous bones and were characterized by a high degree of bone resorption and alterations in OS.