Development a monoclonal antibody-based enzyme-linked immunosorbent assay for screening carotenoids in eggs

卷:202

Peng, Dapeng ; Liao, Feng ; Pan, Yuanhu ; Chen, Dongmei ; Liu, Zhenli ; Wang, Yulian ; Yuan, Zonghui

Huazhong Agr Univ

关键词: Carotenoids;    Monoclonal antibody;    Indirect competitive enzyme linked immunosorbent assay;    Eggs;   

DOI  :  10.1016/j.foodchem.2016.01.123

学科分类：食品科学和技术

【 摘 要 】

In this study, a monoclonal antibody (mAb) with broad-specificity against several carotenoid analogs with equal or similar efficacy was prepared. The obtained mAb C11, with the IgG1 isotype, showed cross-reactivity (CR) with canthaxanthin (100%), beta-ionone acid (140.4%), beta-carotene (92.9%), capsanthin (90.1%), beta-apo-8'-carotenal (92.7%), and xanthophyll (95.8%). Using the mAb C11, a highly sensitive and inexpensive indirect competitive enzyme linked immunosorbent assay (ic-ELISA) was developed with a simple sample preparation procedure for the simultaneous detection of these carotenoid compounds in eggs. The limit of detection of the various carotenoids ranged from 1.31 mg kg(-1) to 1.48 mg kg(-1). Recoveries from egg yolks spiked with the above carotenoids ranged from 91.8% to 113.3%, with coefficients of variation (CVs) of less than 14.8%. These results suggest that the developed ic-ELISA is a sensitive, specific, accurate, and inexpensive method that is suitable for the screening of carotenoid residues in routine monitoring. (c) 2016 Published by Elsevier Ltd.