4-Hydroxyderricin, as a PPAR gamma Agonist, Promotes Adipogenesis, Adiponectin Secretion, and Glucose Uptake in 3T3-L1 Cells

卷:51

Li, Yongjia ; Goto, Tsuyoshi ; Yamakuni, Kanae ; Takahashi, Haruya ; Takahashi, Nobuyuki ; Jheng, Huei-Fen ; Nomura, Wataru ; Taniguchi, Masahiko ; Baba, Kimiye ; Murakami, Shigeru ; Kawada, Teruo

Kyoto Univ

关键词: 4-Hydroxyderricin;    Angelica keiskei;    Adipocyte;    Adiponectin;    Glucose uptake;    PPAR gamma;   

DOI  :  10.1007/s11745-016-4154-9

学科分类：食品科学和技术

【 摘 要 】

Adipocyte differentiation plays a pivotal role in maintaining the production of small-size adipocytes with insulin sensitivity, and impaired adipogenesis is implicated in insulin resistance. 4-Hydroxyderricin (4-HD), a phytochemical component of Angelica keiskei, possesses diverse biological properties such as anti-inflammatory, antidiabetic, and antitumor. In the present study, we investigated the effects of 4-HD on adipocyte differentiation. 4-HD promoted lipid accumulation in 3T3-L1 cells, upregulated both peroxisome proliferator-activated receptor (PPAR)-gamma mRNA and protein expression, and acted as a ligand for PPAR gamma in the luciferase assay. Moreover, 4-HD increased the mRNA and protein expression levels of adiponectin. Additionally, it promoted insulin-dependent glucose uptake into 3T3-L1 adipocytes and increased Akt phosphorylation and glucose transporter (GLUT) 4 mRNA expression. In summary, these findings suggest that 4-HD, which promoted adipogenesis and insulin sensitivity in 3T3-L1 cells, might be a phytochemical with potent insulin-sensitizing effects.