Magnetic nanoparticles based dispersive micro-solid-phase extraction as a novel technique for the determination of estrogens in pork samples

卷:204

Wang, Juan ; Chen, Zhiyan ; Li, Zhiming ; Yang, Yaling

Kunming Univ Sci & Technol

关键词: Magnetic nanoparticles based dispersive micro-solid-phase extraction;    Fe3O4@caprylic acid NPs;    CTAB;    17 beta-Estradiol;    Estrone;    Diethylstilbestrol;    HPLC;   

DOI  :  10.1016/j.foodchem.2016.02.016

学科分类：食品科学和技术

【 摘 要 】

A simple and rapid magnetic nanoparticles (MNPs) based dispersive micro-solid-phase extraction (D-mu-SPE) method coupled with HPLC-DAD has been proposed for simultaneous determination of three estrogens (17 beta-estradiol (E2), estrone (E1) and diethylstilbestrol (DES)) in pork samples. In this paper, the synthesis of cetyltrimethyl ammonium bromide (CTAB)-coated Fe3O4@caprylic acid NPs as an efficient sorbent for its high surface area, excellent adsorption capacity, good dispersion ability and high superparamagnetic property was successfully applied to adsorb estrogens. Vortex was used to enhance mass transfer rate as it provided mild and effective mixing of sample solution and increased the contact between analytes and MNPs. The parameters affecting the extraction efficiency were investigated in detail. The dosages of sorbent and eluate are 100 mu L and 500 mu L, respectively. The extraction equilibrium was achieved within 2 min and the MNPs can be reused. The proposed technique provided high recoveries (93.3-106.7%), good linearity (0.9993-0.9999), low LODs (0.021-0.033 ng mL(-1)) and repeatability (RSD% = 1.87-2.92). (C) 2016 Elsevier Ltd. All rights reserved.