Partial Enteral Nutrition Mitigated Ischemia/Reperfusion-Induced Damage of Rat Small Intestinal Barrier

卷:8

Wu, Chao ; Wang, Xinying ; Jiang, Tingting ; Li, Chaojun ; Zhang, Li ; Gao, Xuejin ; Tian, Feng ; Li, Ning ; Li, Jieshou

Nanjing Univ

关键词: partial enteral nutrition;    ischemia-reperfusion injury;    hypoxia-inducible factor;    intestinal epithelial barrier function;   

DOI  :  10.3390/nu8080502

学科分类：食品科学和技术

【 摘 要 】

Background and Aims: This study was designed to investigate a relatively optimum dose of partial enteral nutrition (PEN) which effectively attenuates intestinal barrier dysfunction initiated by ischemia/reperfusion injury (IRI). Methods: In experiment 1, 60 male Sprague-Dawley (SD) rats were subjected to intestinal IRI and assigned to six groups according to the different proportion of EN administrations: namely total parenteral nutrition (TPN or 0% EN), 10% EN, 20% EN, 40% EN, 60% EN, and total enteral nutrition (TEN or 100%) groups, the deficits of intraluminal calorie were supplemented by PN. In experiment 2, 50 male SD rats were subjected to intestinal IRI and divided into five groups based on the results of experiment 1: TPN, TEN, 20% EN, TPN plus pretreatment with NF-kappa B antagonist 30 min before IRI (TPN+PDTC), and TPN plus pretreatment with HIF-1 alpha antagonist 30 min before IRI (TPN+YC-1) groups. Results: In experiment 1, previous IRI combined with subsequent EN shortage disrupted the structure of intestinal epithelial cell and tight junctions (TJs). While 20% dose of EN had an obviously protective effect on these detrimental consequences. In experiment 2, compared with TPN only, 20% EN exerted a significant protection of barrier function of intestinal epithelium. Analogous results were observed when TPN combined with specific NF-kappa B/HIF-1 alpha inhibitors (PDTC and YC-1). Meanwhile, the expression of NF-kappa B/HIF-1 alpha had a similar trend among the groups. Conclusions: Our findings indicate that 20% EN is the minimally effective dosage of EN which promotes the recovery of intestinal barrier function after IRI in a rat model. Furthermore, we discreetly speculate that this benefit is, at least partly, related to NF-kappa B/HIF-1 alpha pathway expression.