On-cartridge derivatisation using a calixarene solid-phase extraction sorbent for facile, sensitive and fast determination of formaldehyde in beer

卷:211

Deng, Zhifen ; Hu, Kai ; Zhang, Yongming ; Zhao, Wenjie ; Wang, Fei ; Guo, Ling ; Zhang, Wenfen ; He, Juan ; Huang, Yanjie ; Zhang, Shusheng

75 Daxue Rd

关键词: Formaldehyde;    2,4-Dinitrophenylhydrazine;    Beer;    Solid-phase extraction;    Derivatisation;    Tetraazacalix[2]arene[2]triazine sorbent;   

DOI  :  10.1016/j.foodchem.2016.05.069

学科分类：食品科学和技术

【 摘 要 】

This work demonstrates the successful application of an on-cartridge derivatisation procedure for facile, fast and sensitive determination of formaldehyde in beer by HPLC-UV. The derivatisation and solid-phase extraction (SPE) were integrated into a novel calixarene SPE sorbent: tetraazacalix[2]arene[2]triazine bonded silica gel. Specifically, 2,4-dinitrophenylhydrazine was adsorbed onto the sorbent in advance, based on the charge-transfer interaction between the macrocyclic molecule and nitrobenzenes. The method was optimised and validated: under the optimal conditions of derivatisation, SPE and HPLC separation, good linearity was obtained in the range of 0.080-3.2 mu g mL (1) with a correlation coefficient of 0.9939, the limit of detection was 3.0 ng mL (S/N = 3), the limit of quantification was 10 ng mL (S/N = 10), and the recovery level using this method was desirable at 75-84%. The developed method was successfully applied to determine formaldehyde content in real beer samples; the results were in the range of 0.11-1.1 mu g mL (C) 2016 Published by Elsevier Ltd.