Multiclass method for pesticides quantification in honey by means of modified QuEChERS and UHPLC-MS/MS

卷:211

Souza Tette, Patricia Amaral ; da Silva Oliveira, Fabiano Aurelio ; Correa Pereira, Elba Nathalia ; Silva, Gilsara ; de Abreu Gloria, Maria Beatriz ; Fernandes, Christian

Univ Fed Minas Gerais

关键词: Pesticides;    Honey;    QuEChERS;    UHPLC-MS/MS;    Proficiency test;   

DOI  :  10.1016/j.foodchem.2016.05.036

学科分类：食品科学和技术

【 摘 要 】

Bee products can be produced in an environment contaminated by pesticides that can be transported by honey bees to the hive and incorporated into the honey. Therefore, rapid and modern methods to determine pesticide residues in honey samples are essential to guarantee consumers' health. In this study, a simple multiresidue method for the quantification of 116 pesticides in honey is proposed. It involves the use of a modified QuEChERS procedure followed by UHPLC-MS/MS analysis. The method was validated according to the European Union SANCO/12571/2013 guidelines. Acceptable values were obtained for the following parameters: linearity, limit of detection (0.005 mg/kg) and limit of quantification (0.010 and 0.025 mg/kg), trueness (for the four tested levels the recovery assays values were between 70 and 120%), intermediate precision (RSD < 20.0%) and measurement uncertainty tests (< 50.0%). The validated method was applied for determination of 100 honey samples from five states of Brazil. (C) 2016 Elsevier Ltd. All rights reserved.