Effects of chlorogenic acid, caffeine and coffee on components of the purinergic system of streptozotocin-induced diabetic rats

卷:38

Stefanello, Naiara ; Schmatz, Roberta ; Pereira, Luciane Belmonte ; Cardoso, Andreia Machado ; Passamonti, Sabina ; Spanevello, Roselia Maria ; Thome, Gustavo ; Tavares de Oliveira, Giovanna Medeiros ; Kist, Luiza Wilges ; Bogo, Mauricio Reis ; Morsch, Vera Maria ; Chitolina Schetinger, Maria Rosa

Univ Fed Santa Maria

关键词: Caffeine;    Chlorogenic acid;    Coffee;    Diabetes mellitus;    Purinergic system;   

DOI  :  10.1016/j.jnutbio.2016.08.015

学科分类：食品科学和技术

【 摘 要 】

We evaluated the effect of chlorogenic acid (CGA), caffeine (CA) and coffee (CF) on components of the purinergic system from the cerebral cortex and platelets of streptozotocin-induced diabetic rats. Animals were divided into eight groups: control animals treated with (I) water (WT), (II) CGA (5 mg/kg), (III) CA (15 mg/kg) and (IV) CF (0.5 g/kg), and diabetic animals treated with (V) WT, (VI) CGA (5 mg/kg), (VII) CA (15 mg/kg) and (VIII) CF (0.5 g/kg). Our results showed an increase (173%) in adenosine monophosphate (AMP) hydrolysis in the cerebral cortex of diabetic rats. In addition, CF treatment increased adenosine diphosphate (ADP) and AMP hydrolysis in group VIII synaptosomes. Platelets showed an increase in ectonucleotidase activity in group V, and all treatments reduced the increase in adenosine triphosphate and ADP hydrolysis. Furthermore, there was an increase in platelet aggregation of 72% in the diabetic rats, and CGA and CF treatment reduced platelet aggregation by nearly 60% when compared to diabetic rats. In this context, we can suggest that CGA and CF treatment should be considered a therapeutic and scientific target to be investigated in diseases associated with hyperglycemia. (C) 2016 Elsevier Inc. All rights reserved.