Dietary calcium supplementation in adult rats reverts brown adipose tissue dysfunction programmed by postnatal early overfeeding

卷:39

Santos Conceicao, Ellen Paula ; Moura, Egberto Gaspar ; Oliveira, Elaine ; Guarda, Deysla Sabino ; Figueiredo, Mariana Sarto ; Quitete, Fernanda Torres ; Calvino, Camila ; Miranda, Rosiane Aparecida ; Freitas Mathias, Paulo Cezar ; Manhaes, Alex Christian ; Lisboa, Patricia Cristina

Univ Estado Rio de Janeiro

关键词: Small litter;    Overnutrition;    Obesity;    Autonomic function;    Calcium supplementation;    Rat;   

DOI  :  10.1016/j.jnutbio.2016.09.013

学科分类：食品科学和技术

【 摘 要 】

Brown adipose tissue (BAT) dysfunction is associated with obesity and its comorbidities, such as hypertension, and the improvement of BAT function seems important for obesity management. Here we investigated the effects of dietary calcium supplementation on BAT autonomic nerve activity, sympathoadrenal function and cardiovascular parameters in adult obese rats that were raised in small litters (SL group). Three days afterbirth, SL litters were adjusted to three pups to induce early overfeeding. The control group remained with 10 pups/litter until weaning (NL group). At PN120, the SL group was randomly divided into the following: rats fed with standard chow (SL) and rats fed with dietary calcium carbonate supplementation (SL-Ca, 10g/kg chow). Animals were killed either at PN120 or PN180. At both ages, SL rats had higher BAT autonomic nervous system activity, mass and adipocyte area, as well as increased heart rate and blood pressure (systolic and diastolic); 2 months of calcium supplementation normalized these parameters. At PN180 only, UCP1 and TR beta 1 in BAT were decreased in SL rats. These changes were also prevented by calcium treatment. Also at PN180, the SL group presented higher tyrosine hydroxylase and adrenal catecholamine contents, as well as lower hypothalamic POMC and MC4R contents. Calcium supplementation did not revert these alterations. Thus, we demonstrated that dietary calcium supplementation was able to improve cardiovascular parameters and BAT thermogenesis capacity in adult animals that were early overfed during lactation. (C) 2016 Elsevier Inc. All rights reserved.