Multi-class method for the determination of nitroimidazoles, nitrofurans, and chloramphenicol in chicken muscle and egg by dispersive-solid phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry

卷:217

Zhang, Zhiwen ; Wu, Yuping ; Li, Xiaowei ; Wang, Yingyu ; Li, Hui ; Fu, Qin ; Shan, Yawen ; Liu, Tianhe ; Xia, Xi

China Agr Univ

关键词: Nitroimidazoles;    Nitrofurans;    Chloramphenicol;    Dispersive-solid phase extraction;    Ultra-high performance liquid chromatography;    Tandem mass spectrometry;   

DOI  :  10.1016/j.foodchem.2016.08.097

学科分类：食品科学和技术

【 摘 要 】

This study describes the development of a multiresidue method for the efficient identification and quantification of nitroimidazoles, nitrofurans, and chloramphenicol in chicken and egg. After derivatization of nitrofuran metabolites, dispersive-solid phase extraction was used for the extraction of target analytes. An optimization strategy involved the selection of sorbents and extraction solutions for dispersive-solid phase extraction in order to achieve acceptably high recoveries and reduce co-extractives in the final extracts. Analytes were determined by ultra-high performance liquid chromatography-tandem mass spectrometry, in one single injection with a chromatographic run time of 7.5 min. Mean recoveries ranged from 86.4% to 116.7% and interday precision was lower than 18%. The limits of quantification were between 0.1 and 0.5 mu g/kg, which were satisfactory to support surveillance monitoring. Finally, the method was applied to real samples, and metabolite of furazolidone, metronidazole and its metabolite, dimetridazole and its metabolite were detected in both chicken and egg samples. (C) 2016 Elsevier Ltd. All rights reserved.