Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity

卷:218

Wang, Yongqiang ; Gao, Yujie ; Ding, Hui ; Liu, Shejiang ; Han, Xu ; Gui, Jianzhou ; Liu, Dan

Tianjin Univ

关键词: Response surface methodology;    Flavonoids;    Moringa oleifera leaf;    Subcritical ethanol extraction;    Antioxidant property;   

DOI  :  10.1016/j.foodchem.2016.09.058

学科分类：食品科学和技术

【 摘 要 】

A large-scale process to extract flavonoids from Moringa oleifera leaf by subcritical ethanol was developed and HPLC-MS analysis was conducted to qualitatively identify the compounds in the extracts. To optimize the effects of process parameters on the yield of flavonoids, a Box-Behnken design combined with response surface methodology was conducted in the present work. The results indicated that the highest extraction yield of flavonoids by subcritical ethanol extraction could reach 2.60% using 70% ethanol at 126.6 degrees C for 2.05 h extraction. Under the optimized conditions, flavonoids yield was substantially improved by 26.7% compared with the traditional ethanol reflux method while the extraction time was only 2 h, and obvious energy saving was observed. FRAP and DPPH' assays showed that the extracts had strong antioxidant and free radical scavenging activities. (C) 2016 Elsevier Ltd. All rights reserved.