Suppression of CellWall Degrading Enzymes and their Encoding Genes in Button Mushrooms (Agaricus bisporus) by CaCl2 and Citric Acid

卷:72

Khan, Zia Ullah ; Li Jiayin ; Khan, Nasir Mehmood ; Mou, Wangshu ; Li, Dongdong ; Wang, Yansheng ; Feng, Simin ; Luo, Zisheng ; Mao, Linchun ; Ying, Tiejin

Zhejiang Univ

关键词: Button mushroom;    Chemical treatment;    Texture;    Cell wall degrading enzymes;   

DOI  :  10.1007/s11130-016-0588-8

学科分类：食品科学和技术

【 摘 要 】

Fresh button mushrooms (Agaricus bisporus) were harvested and treated with a solution of 1.5% CaCl2 + 0.5% citric acid and stored for 16 days at 12 degrees C. The effects of this treatment on firmness, weight, color, cell wall compositions (cellulose and chitin) and cell wall degrading enzymes (cel1ulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase) were investigated during post-harvest storage. The expressions of major genes (Cel1, Glu1, Chi1 and PAL1) involved in cell wall degradation during post-harvest storage were also monitored. The results revealed that the post-harvest chemical treatment maintained better firmness, weight, color and inhibited cellulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase activities. These findings showed that the down-regulation of cell wall degrading enzymes is a possible mechanism that delays the softening of button mushrooms by the application of combined chemical treatment.