Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model

卷:56

Kubatka, Peter ; Kello, Martin ; Kajo, Karol ; Kruzliak, Peter ; Vybohova, Desanka ; Mojzis, Jan ; Adamkov, Marian ; Fialova, Silvia ; Veizerova, Lucia ; Zulli, Anthony ; Pec, Martin ; Statelova, Dagmar ; Grancai, Daniel ; Busselberg, Dietrich

Comenius Univ

关键词: Mammary carcinogenesis;    Rat;    Oregano;    Cancer stem cells;    Angiogenesis;    Apoptosis;    Cell proliferation;    MCF-7 cells;   

DOI  :  10.1007/s00394-016-1181-5

学科分类：食品科学和技术

【 摘 要 】

Purpose There has been a considerable interest in the identification of natural plant foods for developing effective agents against cancer. Thus, the anti-tumour effects of oregano in the in vivo and in vitro breast cancer model were evaluated. Methods Lyophilized oregano (ORE) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration. At autopsy, mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation in MCF-7 cells was carried out. Results Low-dose ORE suppressed tumour frequency by 55.5 %, tumour incidence by 44 %, and tumour volume by 44.5 % compared to control animals. Analysis of rat tumour cells showed Ki67, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase after low-dose ORE treatment. High-dose ORE lengthened tumour latency by 12.5 days; moreover, Bcl-2, VEGFR-2, CD24, and EpCAM expression decrease and caspase-3 expression increase in carcinoma cells were observed. Histopathological analysis revealed a decrease in the ratio of high-/low-grade carcinomas in both treated groups. In vitro studies showed that ORE decreased survival and proliferation of MCF-7 cells. In ORE-treated MCF-7 cells, an increase in cells expressing sub-G(0)/G(1) DNA content and an increase in the percentage of annexin V/PI positive MCF-7 cells were observed. In vitro, both caspase-dependent and possible non-caspase-dependent apoptotic pathways were found. The deactivation of anti-apoptotic activity of Bcl-2, a decrease in mitochondrial membrane potential, and the activation of mitochondrial apoptosis pathway were observed in the ORE-treated MCF-7 cells. Conclusions Our results demonstrate, for the first time, a distinct tumour-suppressive effect of oregano in the breast cancer model.