Development of a rapid and sensitive immunosensor for the detection of bacteria

卷:221

Verdoodt, Niels ; Basso, Caroline R. ; Rossi, Bruna F. ; Pedrosa, Valber A.

UNESP Botucatu

关键词: Immunosensor;    Bacteria;    Naked eye detection;   

DOI  :  10.1016/j.foodchem.2016.10.102

学科分类：食品科学和技术

【 摘 要 】

The presence of Gram-positive bacteria in foodstuffs is a chronic worldwide problem. Here, we present a cheap and simple colorimetric method for the detection of Lactobacillus species (spp.) and Staphylococcus aureus (S. aureus) using gold nanoparticles (AuNP) modified with monoclonal anti-Gram-positive bacteria to produce an immune-sensor. Detection is based on the fact that antibody-conjugated AuNPs can readily identify Gram-positive bacteria through antibody-antigen recognition, which results in a color change of AuNPs upon aggregation. The detection limit was 105 CFU/ml in pure culture for Lactobacillus spp. and 120 CFU/ml in pure culture for S. aureus. The method was applied successfully for detection of bacteria in samples of sugar cane, and agreed well with values obtained using other methods. These results suggested that the detection system could be used for the quantitative analysis of Gram-positive bacteria and might be applied potentially by the food industry. (C) 2016 Elsevier Ltd. All rights reserved.