Controlling the potential gastrointestinal fate of beta-carotene emulsions using interfacial engineering: Impact of coating lipid droplets with polyphenol-protein-carbohydrate conjugate

卷:221

Liu, Fuguo ; Ma, Cuicui ; Zhang, Ruojie ; Gao, Yanxiang ; McClements, David Julian

China Agr Univ

关键词: Polyphenol-protein-carbohydrate conjugate;    Nanoemulsions;    Physicochemical properties;    In vitro digestion;    Confocal laser scanning microscopy;   

DOI  :  10.1016/j.foodchem.2016.10.057

学科分类：食品科学和技术

【 摘 要 】

The impact of interfacial coatings comprised of polyphenol-protein-carbohydrate conjugates on the properties of nutraceutical-fortified lipid droplets during digestion was investigated. Surface-active chlorogenic acid-lactoferrin-polydextrose (CA-LF-PD) conjugate was synthesized as emulsifier to stabilize lipid droplets in beta-carotene-enriched oil-in-water emulsions. Changes in droplet size, charge, and microstructure were monitored as beta-carotene emulsions were passed through a simulated gastrointestinal tract model (mouth, stomach, small intestine). LF-coated droplets were unstable to flocculation at pH 8.0-9.0, due to the reduction in electrostatic repulsion, but CA-LF-PD conjugate-coated droplets were stable. Emulsions stabilized by ternary conjugate had better stability to droplet aggregation under simulated GIT conditions than other systems, which increased beta-carotene bioaccessibility. The importance of including an oral phase in the simulated GIT model was also demonstrated. The ternary conjugate-stabilized emulsions developed in this study have potential applications as protectors and carriers of hydrophobic drugs, supplements and nutraceuticals. (C) 2016 Elsevier Ltd. All rights reserved.