Enhancing the antimicrobial activity of D-limonene nanoemulsion with the inclusion of epsilon-polylysine

卷:221

Zahi, Mohamed Reda ; El Hattab, Mohamed ; Liang, Hao ; Yuan, Qipeng

Beijing Univ Chem Technol

关键词: epsilon-Polylysine;    D-limonene;    Antimicrobial activity;    Nanoemulsion;   

DOI  :  10.1016/j.foodchem.2016.10.037

学科分类：食品科学和技术

【 摘 要 】

The objective of this research was to investigate the synergism between epsilon-polylysine and D-limonene and develop a novel nanoemulsion system by merging the positive effect of these two antimicrobial agents. Results from the checkerboard method showed that epsilon-polylysine and D-limonene exhibit strong synergistic and useful additive effects against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Saccharomyces cerevisiae. In addition, D-limonene nanoemulsion with the inclusion of epsilon-polylysine was successfully prepared by high pressure homogenizer technology. Its antimicrobial efficiency was compared with pure D-limonene nanoemulsion by measuring the minimal inhibitory concentration, electronic microscope observation and the leakage of the intercellular constituents. The results demonstrated a wide improvement of the antimicrobial activity of D-limonene nanoemulsion following the inclusion of epsilon-polylysine. Overall, the current study may have a valuable contribution to make in developing a more efficient antimicrobial system in the food industry. (C) 2016 Elsevier Ltd. All rights reserved.