DNP and ATP induced alteration in disease development of Phomopsis longanae Chi-inoculated longan fruit by acting on energy status and reactive oxygen species production-scavenging system

卷:228

Lin, Yifen ; Chen, Mengyin ; Lin, Hetong ; Hung, Yen-Con ; Lin, Yixiong ; Chen, Yihui ; Wang, Hui ; Shi, John

Fujian Agr & Forestry Univ

关键词: Longan (Dimocarpus longan Lour.) fruit;    Pericarp browning;    Disease development;    Energy status;    Active oxygen metabolism;    Phomopsis longanae Chi;    2,4-Dinitrophenol (DNP);    Adenosine triphosphate (ATP);   

DOI  :  10.1016/j.foodchem.2017.02.045

学科分类：食品科学和技术

【 摘 要 】

As compared with P. longanae-inoculated longans, DNP treatment for P. longanae-inoculated longans exhibited higher fruit disease index and pericarp browning index, lower ATP amount and energy charge level, lower activities of SOD, CAT and APX, lower amounts of AsA and GSH, lower levels of DPPH radical scavenging activity and reducing power, higher O-2(-center dot) generating rate and MDA amount. However, supply of ATP for P. longanae-inoculated longans showed the contrary effects. These results gave convincing evidence that DNP treatment for accelerating pericarp browning and disease development of harvested longans caused by P. longanae was due to decreases of energy production and ROS scavenging capacity, and increases of O-2(-center dot) accumulation and membrane lipid peroxidation. Whereas, supply of ATP for retarding pericarp browning and disease development of harvested longans caused by P. longanae was due to increases of energy production and ROS scavenging capacity, and reductions of O-2(-center dot) accumulation and membrane lipid peroxidation. (C) 2017 Elsevier Ltd. All rights reserved.