A new real-time PCR method for rapid and specific detection of ling (Molva molva)

卷:228

Taboada, Ledicia ; Sanchez, Ana ; Sotelo, Carmen G.

CSIC

关键词: Seafood authentication;    Fraud;    Real-time PCR;    Ling;    Molva molva;   

DOI  :  10.1016/j.foodchem.2017.01.117

学科分类：食品科学和技术

【 摘 要 】

Seafood fraud - often involving substitution of one species by another - has attracted much attention as it is prevalent worldwide. Whilst DNA analysis has helped to combat this type of fraud some of the methods currently in use are time-consuming and require sophisticated equipment or highly-trained personnel. This work describes the development of a new, real-time PCR TaqMan assay for the detection of ling (Molva molva) in seafood products. For this purpose, specific primers and a minor groove binding (MGB) TaqMan probe were designed to amplify the 81 bp region on the cyt b gene. Efficiency, specificity and cross-reactivity assays showed statistically significant differences between the average Ct value obtained for Molva molva DNA (19.45 +/- 0.65) and the average Ct for non-target species DNA (38.3 +/- 2.8), even with closely related species such as Molva dypterygia (34.9 +/- 0.09). The proposed methodology has been validated with 31 commercial samples. (C) 2017 Elsevier Ltd. All rights reserved.