Synthesis of 1,3-distearoyl-2-oleoylglycerol by enzymatic acidolysis in a solvent-free system

卷:228

Wang, Xiaosan ; Chen, Yang ; Zheng, Liyou ; Jin, Qingzhe ; Wang, Xingguo

Jiangnan Univ

关键词: 1,3-Distearoyl-2-oleoylglycerol;    Acidolysis;    NS40086 lipase;    Anti-blooming agent;    Fractionation;   

DOI  :  10.1016/j.foodchem.2017.01.146

学科分类：食品科学和技术

【 摘 要 】

1,3-Distearoyl-2-oleoylglycerol (SOS) is widely used as a cocoa butter improver and an anti-blooming agent in the chocolate industry. In this study, an effective process was developed to prepare SOS by enzymatic acidolysis. Under optimal reaction conditions (substrate molar ratio of 12, NS40086 loading of 10%, a solvent-free system, 75 degrees C for 4 h), a SOS yield of 70.2% was obtained. Subsequently, two-step purification was applied to purify the crude SOS product. Free fatty acids were completely removed after molecular distillation. After acetone fractionation, the purity of SOS reached 92.2% with an 85.1% recovery. Comparison of various physicochemical properties of purified SOS and a commercial cocoa butter improver showed their properties to be similar. This study provides an effective and sustainable process for the synthesis of SOS product, which is expected to be used as a high-quality cocoa butter improver or an anti blooming agent in the chocolate industry. (C) 2017 Elsevier Ltd. All rights reserved.