Comparative proteomics to reveal muscle-specific beef color stability of Holstein cattle during post-mortem storage

卷:229

Yu, Qianqian ; Wu, Wei ; Tian, Xiaojing ; Jia, Fei ; Xu, Lei ; Dai, Ruitong ; Li, Xingmin

China Agr Univ

关键词: Color stability;    M. longissimuss lumborum;    M. psoas major;    Label-free;    Proteomics;   

DOI  :  10.1016/j.foodchem.2017.03.004

学科分类：食品科学和技术

【 摘 要 】

Label-free strategy was applied to elucidate muscle-specific beef (M. longissimuss lumborum (LL) and M. psoas major (PM)) color stability of Holstein cattle during post-mortem storage at 4 degrees C +/- 1 degrees C. LL showed greater (p < 0.05) redness (a*) value than PM at day 4 and 9 storage, while the proportion of metmyoglobin in PM exhibited a greater increase than in LL muscle. Furthermore, an overabundance of proteins with the functions of antioxidation, protection, and repair in LL were conducive to its color stability, whereas the overabundant proteins/subunits involved in tricarboxylic acid (TCA) cycle and mitochondrial electron transport chain (ETC) in PM indicated greater oxidative metabolism and degradation of ETC complexes, resulting in poor color stability. Bioinformatic analyses indicated that these proteins mainly participated in oxidation-reduction processes, TCA cycle, and ETC processes. All of these results provided a deeper understanding of muscle-specific beef color stability from the perspective of proteomics. (C) 2017 Elsevier Ltd. All rights reserved.