Analytical validation of an ultraviolet-visible procedure for determining lutein concentration and application to lutein-loaded nanoparticles

卷:230

do Prado Silva, Jessica Thais ; da Silva, Anderson Clayton ; Tonin Geiss, Julia Maria ; Hermes de Araujo, Pedro Henrique ; Becker, Daniela ; Bracht, Livia ; Leimann, Fernanda Vitoria ; Bona, Evandro ; Guerra, Gustavo Petri ; Goncalves, Odinei Hess

Fed Univ Technol Parana UTFPR

关键词: Xanthophyll;    UV-Vis;    Nanoparticles;    Nanoprecipitation;    Bioactive quantification;   

DOI  :  10.1016/j.foodchem.2017.03.059

学科分类：食品科学和技术

【 摘 要 】

Lutein is a carotenoid presenting known anti-inflammatory and antioxidant properties. Lutein-rich diets have been associated with neurological improvement as well as reduction of the risk of vision loss due to Age-Related Macular Degeneration (AMD). Micro and nanoencapsulation have demonstrated to be effective techniques in protecting lutein against degradation and also in improving its bioavailability. However, actual lutein concentration inside the capsules and encapsulation efficiency are key parameters that must be precisely known when designing in vitro and in vivo tests. In this work an analytical procedure was validated for the determination of the actual lutein content in zein nanoparticles using ultraviolet -visible spectroscopy. Method validation followed the International Conference on Harmonisation (ICH) guidelines which evaluate linearity, detection limit, quantification limit, accuracy and precision. The validated methodology was applied to characterize lutein-loaded nanoparticles. (C) 2017 Elsevier Ltd. All rights reserved.