DNA barcoding coupled to HRM analysis as a new and simple tool for the authentication of Gadidae fish species

卷:230

Fernandes, Telmo J. R. ; Costa, Joana ; Oliveira, M. Beatriz P. P. ; Mafra, Isabel

Univ Porto

关键词: DNA barcode;    COI;    Cytb;    High resolution melting analysis;    Real-time PCR;    Codfish;    Gadoid species;   

DOI  :  10.1016/j.foodchem.2017.03.015

学科分类：食品科学和技术

【 摘 要 】

This work aimed to exploit the use of DNA mini-barcodes combined with high resolution melting (HRM) for the authentication of gadoid species: Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Theragra chalcogramma) and saithe (Pollachius virens). Two DNA barcode regions, namely cytochrome c oxidase subunit I (COI) and cytochrome b (cytb), were analysed in silico to identify genetic variability among the four species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis. The cytb mini-barcode enabled best discrimination of the target species with a high level of confidence (99.3%). The approach was applied successfully to identify gadoid species in 30 fish containing foods, 30% of which were not as declared on the label. Herein, a novel approach for rapid, simple and cost-effective discrimination/clustering, as a tool to authenticate Gadidae fish species, according to their genetic relationship, is proposed. (C) 2017 Elsevier Ltd. All rights reserved.