BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain

Stem cells international

Yihong Chen¹  Min Wang²  Zhengliang Gao³  Qingqing Chen³  Hua He³  Liming Yu³  Yuehua Liu³  Xinxin Han³  Guangming Wang⁴  Lixia Lu⁴  Jie Ren⁴  Li Chen⁴  Ying Zhang⁵  Haibin Tian⁵ 

[1] Changzheng Hospital, Second Hospital Affiliated with Second Military Medical University, Shanghai 200003, China, changzheng.h.yynet.cn;School of Medicine, Jiaxing University, Zhejiang 314001, China, zjxu.edu.cn;Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, China, fudan.edu.cn;Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, China, tongji.edu.cn;Tongji University Advanced Institute of Translational Medicine, Shanghai 200092, China

DOI  :  10.1155/2017/7012405

学科分类：生物技术

来源: Hindawi Publishing Corporation

【 摘 要 】

Monkeys are much closer to human and are the most common nonhuman primates which are used in biomedical studies. Neural progenitor cells can originate from the hippocampus of adult monkeys. Despite a few reports, the detailed properties of monkey neural stem cells (NSCs) and their responses to cytokine are still unclear. Here, we derive NSCs from an adult monkey brain and demonstrate that BMP4 inhibits cell proliferation and affects cell morphology of monkey NSCs. Combined treatment of BMP4 and LIF or RA and Forskolin represses the proliferation of monkey NSCs. We also show that BMP4 may promote monkey NSC quiescence. Our study therefore provides implications for NSC-based cell therapy of brain injury in the future.

【 授权许可】

CC BY