Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

Stem cells international

Hua He¹  Yihong Chen¹  Min Wang²  Ying Zhang³  Liming Yu³  Li Chen³  Yuehua Liu³  Xinxin Han³  Zhongliang Liu⁴  Jie Ren⁴  Xinyu Hu⁴  Daiyu Hu⁴  Zhengliang Gao⁵  Xiaoqing Zhang⁵ 

[1] Changzheng Hospital, Second Affiliated Hospital of Second Military Medical University, Shanghai 200003, China, changzheng.h.yynet.cn;School of Medicine, Jiaxing University, Zhejiang 314001, China, zjxu.edu.cn;Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, China, fudan.edu.cn;Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, China, tongji.edu.cn;Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Tongji University Advanced Institute of Translational Medicine, Shanghai 200092, China, tongji.edu.cn

DOI  :  10.1155/2017/9405204

学科分类：生物技术

来源: Hindawi Publishing Corporation

【 摘 要 】

Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.

【 授权许可】

CC BY