Transcript Polymorphism Rates in Soybean Seed Tissue Are Increased in a Single Transformant of Glycine max

International Journal of Plant Genomics

Adam M. Whaley¹  Jessica A. Schlueter¹  Kevin C. Lambirth²  Kenneth L. Bost³  Kenneth J. Piller³ 

[1] Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA, uncc.edu;Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, North Carolina Research Campus, Kannapolis, NC 28081, USA, uncc.edu;Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA, uncc.edu

DOI  :  10.1155/2016/1562041

学科分类：生物科学（综合）

来源: Hindawi Publishing Corporation

【 摘 要 】

Transgenic crops have been utilized for decades to enhance agriculture and more recently have been applied as bioreactors for manufacturing pharmaceuticals. Recently, we investigated the gene expression profiles of several in-house transgenic soybean events, finding one transformant group to be consistently different from our controls. In the present study, we examined polymorphisms and sequence variations in the exomes of the same transgenic soybean events. We found that the previously dissimilar soybean line also exhibited markedly increased levels of polymorphisms within mRNA transcripts from seed tissue, many of which are classified as gene expression modifiers. The results from this work will direct future investigations to examine novel SNPs controlling traits of great interest for breeding and improving transgenic soybean crops. Further, this study marks the first work to investigate SNP rates in transgenic soybean seed tissues and demonstrates that while transgenesis may induce abundant unanticipated changes in gene expression and nucleotide variation, phenotypes and overall health of the plants examined remained unaltered.

【 授权许可】

CC BY