In Vitro Evaluation of Essential Oils Derived from Piper nigrum (Piperaceae) and Citrus limonum (Rutaceae) against the Tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

Biochemistry research international

Helena C. Castro¹  Evelize Folly das Chagas²  Jéssica Meloni⁶  Maria Júlia Nunes⁶  Rafaelle Vinturelle⁶  Leandro Rocha⁷  Viviane Lione⁸  Itabajara S. Vaz⁹  Jeane Nogueira⁹  Camila Mattos⁹ 

[1]Bellarome Aromoterapia, Centro, Rio de Janeiro, RJ, Brazil

[2]Centro Universitário Augusto Motta (UNISUAM), Rio de Janeiro, RJ, Brazil, unisuam.edu.br

[3]Centro de Biotecnologia e Faculdade de Veterinária, Universidade de Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil, ufrgs.br

[4]Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil, ufrj.br

[5]Instituto Nacional de Ciências e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil

[6]Laboratório de Estudos em Pragas e Parasitos, Universidade Federal Fluminense, IB, Departamento de Biologia Celular e Molecular–GCM, Niterói, RJ, Brazil, uff.br

[7]Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, RJ, Brazil, uff.br

[8]Programa de Pós-Graduação em Ciências Aplicadas a Produtos para Saúde, Departamento de Farmácia e Administração Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Doutor Mário Viana 523, 24241-000 Niterói, RJ, Brazil, uff.br

[9]Programa de Pós-Graduação em Ciências e Biotecnologia, Universidade Federal Fluminense, Niterói, RJ, Brazil, uff.br

DOI  :  10.1155/2017/5342947

学科分类：生物化学/生物物理

来源: Hindawi Publishing Corporation

【 摘 要 】

The present research aimed to study the chemical composition and acaricidal activity of Citrus limonum and Piper nigrum essential oils against the cattle tick Rhipicephalus microplus. GC-MS analysis of C. limonum essential oil showed limonene (50.3%), ?-pinene (14.4%), and ?-terpinene (11.7%) as the major components; P. nigrum oil was mainly composed of ?-caryophyllene (26.2%), ?-ocymene (5.8%), and ?-pinene (5.5%). Acaricide activity was evaluated at concentrations of 2.5, 5.0, and 10.0%?(v/v) of each plant oil, as well as 1?:?1 combination of both oils (5%?:?5%, 2.5%?:?2.5%, and 1.25%?:?1.25% each), by immersing engorged R. microplus females for one minute. The LC90 of oils from C. limonum, P. nigrum, and the combination were 4.9%, 14.8%, and 5.1%, respectively. C. limonum essential oil caused 100% mortality of engorged females at the highest concentration (10%). P. nigrum essential oil inhibited egg-laying by up to 96% in a concentration-dependent manner, suggesting it reduces tick fecundity. When combined, the oils presented toxicity as to C. limonum oil alone, but with stronger inhibition of oviposition (5%?:?5%), indicating a possible additive effect against R. microplus. The present data provide support for further investigation of novel natural products to control bovine tick infestations.

【 授权许可】

CC BY