Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence

Biochemistry research international

Nidhi Shrivastava¹  Pramod Kumar Singh²  Sameer S. Bhagyawant²  Neha Gupta² 

[1]Department of Bioscience & Biotechnology, Banasthali University, Banasthali 304022, India, banasthali.org

[2]School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, India, jiwaji.edu

DOI  :  10.1155/2016/3136043

学科分类：生物化学/生物物理

来源: Hindawi Publishing Corporation

【 摘 要 】

In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170?kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.

【 授权许可】

CC BY