Scanless multitarget-matching multiphoton excitation fluorescence microscopy

Journal of Innovative Optical Health Sciences

关键词: Multitarget-matching;    multiphoton microscopy;    SLM;    temporal focusing;   

DOI  :  10.1142/S1793545817500134

学科分类：医学（综合）

来源: World Scientific Publishing Co. Pte. Ltd.

【 摘 要 】

Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved. The SLM shaped an incoming mode-locked, near-infrared Ti:sapphire laser beam into an excitation pattern with addressable shapes and sizes that matched the samples of interest in the field of view. Temporal and spatial focusing were simultaneously realized by combining an objective lens and a blazed grating. The fluorescence signal from illuminated areas was recorded by a two-dimensional sCMOS camera. Compared with a conventional temporal focusing multiphoton microscope, our microscope achieved effective use of the laser power and decreased photodamage with higher axial resolution.

【 授权许可】

Unknown