The Journal of Veterinary Medical Science | |
Histochemical properties of bovine and ovine mammary glands during fetaldevelopment | |
Akane SATO1  Kazuhiro SANBE2  Atchalalt KHURCHABILIG3  Rika FUKUMORI3  Asuka HARA3  Atsushi HIRAO4  Yoshikazu NAGAO5  Tomoyuki ABE6  Mio YAMAGUCHI6  Borjigin SARANTONGLAGA6  Kazuko OGATA6  Hiromichi AYAKAWA6  Shoei SUGITA6  | |
[1] Animal Research Center, Agricultural ResearchDepartment, Hokkaido Research Organization, Hokkaido 081-0038,Japan;Basic Science related to Nursing, School of Nursing,Jichi Medical University, Tochigi 329-0498, Japan;Department of Animal Production Science, UnitedGraduate School of Agricultural Science, Tokyo University of Agriculture andTechnology, Tokyo 183-8509, Japan;Division of Regenerative Medicine, Jichi MedicalUniversity, Tochigi 329-0498, Japan;National Livestock Breeding Center, Fukushima 961-8511,Japan;University Farm, Faculty of Agriculture, UtsunomiyaUniversity, Tochigi 321-4415, Japan | |
关键词: α-SMA; bovine; CK18; fetal mammary gland; ovine; | |
DOI : 10.1292/jvms.17-0584 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
In order to obtain more information on the development of bovine and ovine fetal mammaryglands, a series of mammary glands from fetuses of different ages were analyzed. A totalof 16 bovine fetuses with curved crown rump lengths ranging from 12 cm (80 days) to 75âcm(240 days) and 15 ovine fetuses ranging from 55 days to 131 days were examined. We usedhematoxylin and eosin stain and Oil-Red-O stain to analyze the developmental andmorphogenetic processes of mammary glands. In addition, we used immunohistochemicalstaining to determine the pattern of expression of cytokeratin 18 (CK18) during luminalepithelial differentiation, α-smooth-muscle actin (α-SMA) for myoepithelialdifferentiation, Ki-67 for cell proliferation, and estrogen receptor α (ERα). Our analyzesshowed (a) The primary mammary duct begin to proliferate in a lengthwise within the teatat 90 days in bovine fetuses and 63 days in ovine fetus; (b) luminal epithelial cells andmyoepithelial cells appeared from 90 days in bovine fetuses and 63 days in ovine fetus;(c) proliferation of epithelial cells appeared to coincide with the development of theprimary and secondary ducts; and (d) ERα was not found in the fetal mammary gland, butadipocytes showed the presence of ERα. Overall, these results indicate that the sequenceof events in the prenatal development of the mammary gland of sheep is similar to that ofcattle.
【 授权许可】
Unknown
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