In vitro propagation of damask rose(Rosa damascenacv. Ispahan)

Annals of Biological Research

Farzaneh Tabesh¹ 

关键词: Farzaneh Tabesh;    Maryam Jafarkhani Kermani;    Seyed Mojtaba Khayam Nekuie;    Amir Mousavi;    Ahmad Khalighi;    Damask rose (Rosa damascenacv.Ispahan);    In vitro propagation;    Van der Salm (VS) Medium;    Particular rose medium;    Shoot proliferation;    Root initiation;    Phloroglucinol;   

DOI  :  

学科分类：生物科学（综合）

来源: Scholars Research Library

【 摘 要 】

This research was performed to investigate interactive effects ofplant growth regulators on proliferation and rooting of Rosa damascena cv. Ispahan in Van der Salm (VS) medium,supplemented with 100 mg/l phloroglucinol. For proliferation,BAP (6-Benzylaminopurin) (0, 2, 4 and 8 μM) and Zeatin(4.5 and 9 μM) separately or in combination with 1 μM NAA(1-Naphtalene acetic acid) in VSmedium were used.Rooting was initiated in ½strength VS media supplemented with combinations of NAA (0, 0.5, 1, 1.5 μM) and IBA(Indole-3-butyric acid) (0, 0.5, 1, 1.5 μM) in liquid or agar-gelled (with or without 3 g/l activated charcoal) containing 100 mg/lphloroglucinol. Results were recorded after five weeks. All experiments were analyzed in completely random design with fivereplications and comparison of means was conducted using Duncan Test. The best proliferation was observed in the medium containing 4μM BAPthat was accompanied by maximum number of axillary shoots (2.28), maximum length of axillary shoots (10.40 mm) and maximum number of leaves per axillary shoots (4.27). At rooting stage, the liquid medium consisting of 0.5 μM NAA + 1.5μM IBA resulted in the highest percent of rooting (100%), the maximum average root length (17.66 mm) and the maximum height of the rooted plantlets (59.2mm). Rooted plantlets were successfully acclimatized in pots containing 1:1 (v/v) peat moss and perlite in a mist greenhouse.

【 授权许可】

CC BY