Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA

The Journal of Veterinary Medical Science

Hongmei CHEN¹  Cuiteng CHEN¹  Longfei CHENG¹  Guanghua FU¹  Yu HUANG¹  Shaohua SHI¹  Rongchang LIU¹  Chunhe WAN¹  Ronghui LU¹  Jiansheng LIN¹  Qiuling FU¹ 

[1] Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou 350013, China

关键词: duck;    indirect ELISA;    Pasteurella multocida;    reservoir;    rOmpH protein;   

DOI  :  10.1292/jvms.17-0124

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant outer-membrane protein H (rOmpH) for detection of anti-P. multocida antibodies in serum to determine their prevalence in Chinese ducks. The P. multocida ompH gene was cloned into pET32a, and rOmpH was expressed in Escherichia coli BL21 (DE3). Western blotting revealed that purified rOmpH was recognized by duck antisera against P. multocida, and an indirect ELISA was established. During analysis of serum samples (n=115) from ducks, the rOmpH ELISA showed 95.0% specificity, 100% sensitivity and a 92.0% κ coefficient (95% confidence interval 0.844–0.997) as compared with a microtiter agglutination test. Among 165 randomly selected serum samples, which were collected in 2015 and originated from six duck farms across Fujian Province, China, anti-P. multocida antibodies were detected in 22.42% of apparently healthy ducks, including 25 of 90 sheldrakes (27.8%), eight of 50 Peking ducks (16.0%) and four of 25 Muscovy ducks (16%). Overall, the data suggest that rOmpH is a suitable candidate antigen for the development of an indirect ELISA for detection of P. multocida in ducks; moreover, our results showed that ducks could serve as a potential reservoir for P. multocida infection.

【 授权许可】

Unknown