Sound exposure-induced cytokine gene transcript profile changes in captivebottlenose dolphin (Tursiops truncatus) blood identified by a probe-basedqRT-PCR

The Journal of Veterinary Medical Science

Wei-Cheng YANG¹  I-Hua CHEN¹  Jiann-Hsiung WANG¹  Shih-Jen CHOU¹  I-Fan JEN²  Lien-Siang CHOU³  Myra BLANCHARD⁴  Jeffrey STOTT⁴ 

[1] Department of Veterinary Medicine, National ChiayiUniversity, No. 580, Xinmin Rd., Chiayi, Taiwan (R.O.C.);Farglory Ocean Park, No.189, Yenliao Village, Shoufong, Hualien, Taiwan(R.O.C.);Institute of Ecology and Evolutionary Biology, NationalTaiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, Taiwan(R.O.C.);School of Veterinary Medicine, University ofCalifornia, Davis, One Shields Avenue, Davis, CA 95616, U.S.A.

关键词: cytokine;    dolphin;    immune response;    qRT-PCR;    sound;   

DOI  :  10.1292/jvms.17-0548

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Cetacean health may be potentially affected by anthropogenic sound. We have initiatedinvestigations on the effect of low-frequency underwater sound on immunological genetranscript profiles of captive bottlenose dolphins (Tursiops truncatus)using a probe-based quantitative gene expression assay. Six immunologic genes(IL-2Rα, -4, -10,-12, TNFα and IFNγ) were selected foranalysis using two validated housekeeping genes (PGK1 andHPRT1) as reference genes. Twenty-four blood samples from sixclinically healthy individuals and six blood samples from individuals after soundexposures were available. The gene transcript profile of sound-exposed dolphins wasconsistent with a stress-induced TH2 shift profile as compared to controls.This study may lead to better understanding of the effects of anthropogenic sound onimmune responses of cetaceans.

【 授权许可】

Unknown