Hybrid origin of Asian aspermic Fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold

The Journal of Veterinary Medical Science

Tadashi ITAGAKI¹  Madoka ICHIKAWA-SEKI²  Takuya SHORIKI²  Pannigan CHAICHANASAK³  Kei HAYASHI³  Uday Kumar MOHANTA³ 

[1] Department of Microbiology and Parasitology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh;Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan;Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan

关键词: Asia;    Fasciola;    hybrid;    pepck;    pold;   

DOI  :  10.1292/jvms.17-0406

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages for obtaining a definitive evidence of the hybrid origin of aspermic Fasciola flukes. All the flukes categorized into the aspermic nad1 lineages possessed both the fragment patterns of F. hepatica and F. gigantica (mixed types) in pepck and/or pold. These findings provide clear evidence for the hybrid origin of aspermic Fasciola lineages and suggest that “aspermic Fasciola flukes” should hereafter be called “hybrid Fasciola flukes”.

【 授权许可】

Unknown